![]() ![]() ![]() STICK: Continuous assignment based on line segments While over representation could be regarded as a handicap, it proves to be useful in structure comparison and similarity searches. The net result is over-assignment of secondary structure compared to other methods with approximately 80% of residues being assigned a regular secondary structure class. The β class includes parallel/anti-parallel sheets, bridges, bends and hairpins. The helix class includes 3 10, pi and alpha helices as well as turns that show a helical propensity based on the observation that some helices start and end with tighter, looser and non-backbone hydrogen bonded turns and helices. Predictive Assignment of Linear Secondary Structure Elements (PALSSE) describes three state secondary structure in vector form based on Cα coordinates. This is achieved using a short range Cα distance mask and two angle criteria for each secondary structure. P-SEA assigns secondary structure using Cα coordinates. STRIDE is optimised to meet the visual assignments made by crystallographers. Torsion angles are given propensities according to how close they are to their regions in the Ramachandran plot. The STRuctural IDEntification method (STRIDE) uses empirically derived hydrogen bond energy and phi-psi torrsion angles to assign secondary structure. The continuous output is achieved by running DSSP multiple times with different hydrogen bond thresholds and then imposing a weighted average over the individual DSSP assignments to each residue. DSSPcontĭSSPcont is a variant of DSSP aiming to assign secondary structure in a continuous fashion, thus reflecting the structural variability due to thermal motion. DSSP defines a hydrogen bond where the bond energy is below -0.5 kcal/mol based on a Coulomb approximation of the hydrogen bond energy. DSSPĭictionary of Secondary Structure of Proteins (DSSP) assigns eight state secondary structure using hydrogen bonds alone. Janes, 2010, 2Struc - The Protein Secondary Structure Analysis Server, Biophysical Journal, 98:454a-455) and each of the methods you run. If you use 2Struc and publish your work please cite our paper (Klose, D & R.W. In each reference box you will find a link to the original published work and, where available, web based resources. Please click the purple buttons to access the references. Presented below is a short description of each method applied in 2Struc. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |